Abstract
Abstract
Objectives
Dental caries is a widespread multifactorial disease, caused by the demineralization of hard dental tissues. Susceptibility to dental caries is partially genetically conditioned; this study was aimed at finding an association of selected single nucleotide polymorphisms (SNPs) in genes encoding proteins involved in amelogenesis with this disease in children.
Materials and methods
In this case–control study, 15 SNPs in ALOX15, AMBN, AMELX, KLK4, TFIP11, and TUFT1 genes were analyzed in 150 children with primary dentition and 611 children with permanent teeth with/without dental caries from the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) cohort.
Results
Dental caries in primary dentition was associated with SNPs in AMELX (rs17878486) and KLK4 (rs198968, rs2242670), and dental caries in permanent dentition with SNPs in AMELX (rs17878486) and KLK4 (rs2235091, rs2242670, rs2978642), (p ≤ 0.05). No significant differences between cases and controls were observed in the allele or genotype frequencies of any of the selected SNPs in ALOX15, AMBN, TFIP11, and TUFT1 genes (p > 0.05). Some KLK4 haplotypes were associated with dental caries in permanent dentition (p ≤ 0.05).
Conclusions
Based on this study, we found that although the SNPs in AMELX and KLK4 are localized in intronic regions and their functional significance has not yet been determined, they are associated with susceptibility to dental caries in children.
Clinical relevance
AMELX and KLK4 variants could be considered in the risk assessment of dental caries, especially in permanent dentition, in the European Caucasian population.
Funder
Ministerstvo Zdravotnictví Ceské Republiky
Ministerstvo Školství, Mládeže a Tělovýchovy
Horizon 2020 Framework Programme
Publisher
Springer Science and Business Media LLC
Reference66 articles.
1. American Academy of Peadiatric Dentistry. Policy on early childhood caries (ECC): classifications, consequences, and preventive strategies. 2018 [online]. [cit. 20.1.2021] https://www.aapd.org/media/policies_guidelines/p_eccclassifications.pdf
2. Skeie MS, Raadal M, Strand GV, Espelid I (2006) The relationship between caries in the primary dentition at 5 years of age and permanent dentition at 10 years of age - a longitudinal study. Int J Paediatr Dent 16:152–160. https://doi.org/10.1111/j.1365-263X.2006.00720.x
3. Zemaitiene M, Grigalauskiene R, Andruskeviciene V et al (2017) Dental caries risk indicators in early childhood and their association with caries polarization in adolescence: a cross-sectional study. BMC Oral Health 17:2. https://doi.org/10.1186/s12903-016-0234-8
4. Wang LJ, Tang R, Bonstein T et al (2006) Enamel demineralization in primary and permanent teeth. J Dent Res 85:359–363. https://doi.org/10.1177/154405910608500415
5. Piler P, Kandrnal V, Kukla L et al (2017) Cohort profile: the European Longitudinal Study of Pregnancy and Childhood (ELSPAC) in the Czech Republic. Int J Epidemiol 46:1379–1379f. https://doi.org/10.1093/ije/dyw091
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献