Abstract
AbstractEiseniella neapolitana is a semi-aquatic, diploid earthworm that for many years was related to the cosmopolitan species Eiseniella tetraedra and even considered a subspecies of it. Norealidys andaluciana was described in Spain and is usually synonymized with E. neapolitana. We collected 69 specimens from Italy, Spain, and Cyprus and studied five molecular markers (COI, 16S, 28S, 12S, and ND1) and their morphology to solve this taxonomic problem. Phylogenetic analyses reveal the possible existence of two separate genera confounded under the name Eiseniella, but the study of more molecular markers and species of the genus would be necessary to confirm this. Therefore, the synonymy between Eiseniella and Norealidys is maintained. Various genetic analyses, including species delimitation, confirm the separation between E. neapolitana and E. andaluciana (= N. andaluciana) and excluded that E. neapolitana is a subspecies of E. tetraedra. The resemblance in external appearance despite clear genetic differences of the three species could be explained by convergent adaptation to the aquatic habitat. Despite the expected low haplotype diversity based on the 28S gene, we found a surprisingly high variability in the E. andaluciana (= N. andaluciana) population in Spain. However, its stable predicted secondary structure and its high content of G + C reject the presence of a pseudogene.
Funder
Universidad Complutense de Madrid
Minsterio de Ciencia e Innovación. Gobierno de España
French government
Spanish Government
Ministerio de Ciencia e Innovación
Narodowe Centrum Nauki
Publisher
Springer Science and Business Media LLC
Subject
Ecology, Evolution, Behavior and Systematics
Reference66 articles.
1. Arctander, P. (1995). Comparison of a mitochondrial gene and a corresponding nuclear pseudogene. Proceedings of the Royal Society of London b: Biological Science, 262, 13–19. https://doi.org/10.1098/rspb.1995.0170
2. Balian, E. V., Segers, H., Martens, K., & Lévêque, C. (2007). An introduction to the freshwater animal diversity assessment (FADA) project. In Freshwater Animal Diversity Assessment (pp. 3–8). Springer, Dordrecht.
3. Blakemore, R. J. (2008). Cosmopolitan earthworms – an Eco-Taxonomic Guide to the Species (2nd ed.). VermEcology.
4. Bodenheimer, F. S. (1937). Prodromus faunae Palestinae. Essai sur les éléments zoogeographiques et historiques du sud-ouest du sous-règne Paléarctique. Mémoires Présentés a L’institut D’egypte, 33, 1–286.
5. Černosvitov, L. (1938). The Oligochaeta. In Washbourn R, Jones RF (eds): Report of the Percy Sladen Expedition to Lake Huleh; a contribution to the study of fresh waters of Palestine. Annals and Magazine of Natural History, 11, 535–550.
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