Preparation of a Single-Cell Suspension from Tumor Biopsy Samples for Single-Cell RNA Sequencing-Reference-Cited by-同舟云学术

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Preparation of a Single-Cell Suspension from Tumor Biopsy Samples for Single-Cell RNA Sequencing

Published:2023-08 Issue:4 Volume:175 Page:519-523
ISSN:0007-4888
Container-title:Bulletin of Experimental Biology and Medicine
language:en
Short-container-title:Bull Exp Biol Med

Author:

Frolova A. A.,Gerashchenko T. S.,Patysheva M. R.,Fedorov A. A.,Tsyganov M. M.,Bokova U. A.,Bragina O. D.,Vostrikova M. A.,Garbukov E. Yu.,Cherdyntseva N. V.

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://link.springer.com/content/pdf/10.1007/s10517-023-05898-9.pdf

Reference12 articles.

1. Chen G, Ning B, Shi T. Single-Cell RNA-Seq Technologies and Related Computational Data Analysis. Front. Genet. 2019;10:317. https://doi.org/10.3389/fgene.2019.00317

2. Potter SS. Single-cell RNA sequencing for the study of development, physiology and disease. Nat. Rev. Nephrol. 2018;14(8):479-492. https://doi.org/10.1038/s41581-018-0021-7

3. Ilicic T, Kim JK, Kolodziejczyk AA, Bagger FO, McCarthy DJ, Marioni JC, Teichmann SA. Classification of low quality cells from single-cell RNA-seq data. Genome Biol. 2016;17:29. https://doi.org/10.1186/s13059-016-0888-1

4. Madden RE, Burk D. Production of viable single cell suspensions from solid tumors. J. Natl. Cancer Inst. 1961;841-861.

5. Quatromoni JG, Singhal S, Bhojnagarwala P, Hancock WW, Albelda SM, Eruslanov E. An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells. J. Leukoc. Biol. 2015;97(1):201-209. https://doi.org/10.1189/jlb.5TA0814-373.

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