Enzyme linked immunosorbent assay (ELISA) for the determination of protein-A-Reference-Cited by-同舟云学术

Enzyme linked immunosorbent assay (ELISA) for the determination of protein-A

Published:1986-11-01 Issue:11 Volume:6 Page:933-936
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Considine P. J.¹,Duggan P.²,Eadie A.²

Affiliation:

1. IIRS, Biochemicals Unit, University College, Galway, Ireland

2. Noctech SBD Centre, University College, Galway, Ireland

Abstract

A competitive ELISA for the determination of protein-A concentration in culture supernatants is described. The sensitivity of the assay is 50 ng/ml and it may be used to determine concentrations of protein-A up to 1000 ng/ml. The assay is specific, rapid and suitable for routine screening of protein-A producing microorganisms.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/6/11/933/471923/bsr0060933.pdf

Reference7 articles.

1. Forsgren, A. and Sjoquist, J. (1967).Acta Pathol. Microbiol. Scand. 71:400?406.

2. Forsgren, A. (1970).Infect. and Immun. 2:672?673.

3. Movitz, J. (1976).Eur. J. Biochem. 68:291?299.

4. Lofdahl, A., Guss, B., Uhlen, M., Phillipson, L. and Lindberg, M. (1983).Proc. Natl. Acad. Sci. USA 80:697?701.

5. Fahnestock, S. R. and Fisher K. E. (1986).J. Bacteriol. 165:796?804.

Cited by 9 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Boronate affinity material-based sensors for recognition and detection of glycoproteins;The Analyst;2020

2. A highly sensitive method for detection of protein based on inhibition of Ru(bpy)32+/TPrA electrochemiluminescent system;Electrochimica Acta;2011-08

3. A very sensitive enzyme-linked immunoabsorbent assay to Staphylococcal Protein A in the presence of immunoglobulins;Biochemical and Biophysical Research Communications;2003-04

4. A simple method to quantify staphylococcal protein A in the presence of human or animal IgG in various samples;Journal of Immunological Methods;2000-02

5. Monitoring impurities in biopharmaceuticals produced by recombinant technology;Pharmaceutical Science & Technology Today;1999-02