1. S. S. Tobe, Experientia30, 517 (1974).
2. This colony was started with pupae obtained in July 1973 from the Tsetse Research Laboratory, University of Bristol, Langford, Bristol, England. Flies in our colony are comparable to the Langford colony in terms of pupal weight, longevity, and reproductive capacity.
3. The system used consisted of a 1 mm thick slab of agar laid over a stretched parafilm membrane as described byP. A. Langley, Bull. ent. Res.62, 215–228, and further modified byA. Mews (unpublished) by using a stippled glass plate to hold the artificial meal and by adding 2% glycerol to the agar during its preparation.
4. P. A. Langley andR. W. Pimley, J. Insect Physiol.19, 1097 (1973).
5. P. W. Hochachka andG. N. Somero,Strategies of Biochemical Adaptation (W. B. Saunders Co. Philadelphia, London, Toronto 1973), p. 358.