Affiliation:
1. H.E.J. Research Institute of Chemistry, University of Karachi, Karachi-32, Pakistan
Abstract
Hemoglobin from the cobra snake, Naja naja naja, was isolated and its chains separated on a CM-cellulose column. The separation profile revealed an α and two β chains having the molar proportions of [α]2,[β1]1,[β2]1. The N-terminal amino acid sequence of the intact chains and of the CNBr peptides were carried out. The β2 chain was found to be heterogeneous comprising a minor component amounting to 11%. This later showed changes at two positions 9 and 14 in the first 30 residues sequenced.
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
Reference17 articles.
1. Hunt, L. T., Hurst-Calderone, S. and Dayhoff, M. O. (1978). In:Atlas of Protein Sequence and Structure (M. O. Dayhoff, Ed.), vol. 5, Suppl. 3, pp. 229?249, National Biomedical Research Foundation, Washington, D.C.
2. Duguet, M., Chauvet, J.-P. and Acher, R. (1974).FEBS Lett. 47:333?337.
3. Hilse, K. and Braunitzer, G. (1968).Hoppe-Seyler's. Z. Physiol. Chem. 349:433?450.
4. Leclercq, F., Schnek, A. G., Braunitzer, G., Stangl, A. and Schrank, B. (1981).Hoppe-Seyler's Z. Physiol. Chem. 362:1151?1158.
5. Leclercq, F., Stangl, A., Schrank, B., Vandecasserie, C., Paul, C., Schnek, A. G., Leonis, J. and Braunitzer, G. (1982).Int. J. Peptide Protein Res. 20:337?350.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献