One bout of endurance exercise does not change gene expression or proliferation in a C26 colon carcinoma in immunocompetent mice
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Published:2023-10-16
Issue:19
Volume:149
Page:17361-17369
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ISSN:0171-5216
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Container-title:Journal of Cancer Research and Clinical Oncology
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language:en
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Short-container-title:J Cancer Res Clin Oncol
Author:
Mahnic Nik, Geremia Alessia, Straub Tobias, Zorzato Sabrina, Schönfelder Martin, von Lüttichau Irene, Steiger Katja, Saller Maximilian Michael, Blaauw Bert, Wackerhage HenningORCID
Abstract
Abstract
Purpose
Exercise typically reduces tumour growth, proliferation and improves outcomes. Many of these effects require exercise to change gene expression within a tumour, but whether exercise actually affects gene expression within a tumour has not been investigated yet. The aim of this study was, therefore, to find out whether one bout of endurance exercise alters gene expression and proliferation in a C26 carcinoma in immunocompetent mice.
Methods
BALB/c were injected with C26 colon carcinoma cells. Once the tumours had formed, the mice either ran for 65 min with increasing intensity or rested before the tumour was dissected. The tumours were then analysed by RNA-Seq and stained for the proliferation marker KI67.
Results
One bout of running for 65 min did not systematically change gene expression in C26 carcinomas of BALB/c mice when compared to BALB/c mice that were rested. However, when analysed for sex, the expression of 17, mostly skeletal muscle-related genes was higher in the samples of the female mice taken post-exercise. Further histological analysis showed that this signal likely comes from the presence of muscle fibres from the panniculus carnosus muscle inside the tumours. Also, we found no differences in the positivity for the proliferation marker KI67 in the control and exercise C26 carcinomas.
Conclusion
A bout of exercise did not systematically affect gene expression or proliferation in C26 carcinomas in immunocompetent BALB/c mice.
Funder
Zukunft Gesundheit e.V. AIRC Technische Universität München
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Oncology,General Medicine
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