Author:
Pavlova Elitsa,Stojchevski Radoslav,Avtanski Dimiter
Abstract
Abstract
Purpose
Resistin is an inflammatory cytokine secreted mostly by adipocytes and immune cells that plays a role in the development of insulin resistance, diabetes, and cancer. We hypothesized that resistin’s inflammatory activity influences the free radical and oxidative stress pathways.
Methods
We used human breast carcinogenic (MCF-7 and MDA-MB-231) and non-carcinogenic (MCF-10A) cells in this investigation and correlated the absorbed resistin concentration with the change in oxidative stress (TBARS, carbonated proteins) and antioxidant activity (Antioxidant Capacity, SuperOxideDismutase, CATalase, Glutathione Peroxidase).
Results
Resistin was substantially more effective as a prooxidant at lower (12.5 ng/ml) concentrations, than at higher concentrations (25.0 ng/ml). Vitamin C did not appear to be an effective oxidative stress protector at antioxidant concentrations of 5.10–4 M. Leptin, at 100 ng/ml, did not result in conclusive oxidative stress or antioxidant defence stimulation, as expected.
Conclusion
Taken together, the findings support resistin’s role as a non-oxidative stress marker and a metabolic signaling molecule.
Funder
Bulgarian-American Commission for Educational Exchange FULBRIGHT, Bulgaria
Gerald J. and Dorothy R. Friedman New York Foundation for Medical Research, New York, NY, USA
Publisher
Springer Science and Business Media LLC
Subject
Cancer Research,Oncology,General Medicine