Design, Expression, Purification and Characterization of the Recombinant Immunotoxin 4D5 scFv-TRAIL

Author:

Wang Zhanqing,Chi Lili,Shen Yaling

Funder

National Natural Science Foundation of China

Shanghai Leading Academic Discipline Project

Research Program of State Key Laboratory of Bioreactor Engineering

Publisher

Springer Science and Business Media LLC

Subject

Drug Discovery,Molecular Medicine,Biochemistry,Bioengineering,Analytical Chemistry

Reference28 articles.

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2. Brinkmann U (2000) Recombinant antibody fragments and immunotoxin fusions for cancer therapy. Vivo 14:21

3. Butt TR, Edavettal SC, Hall JP, Mattern MR (2005) SUMO fusion technology for difficult-to-express proteins. Protein Expres Purif 43:1–9

4. Cao Y, Marks JW, Liu Z, Cheung LH, Hittelman WN, Rosenblum MG (2013) Design optimization and characterization of Her2/neu-targeted immunotoxins: comparative in vitro and in vivo efficacy studies. Oncogene 33:429–439

5. Chen X, Zaro JL, Shen W-C (2013) Fusion protein linkers: property, design and functionality. Adv Drug Deliv Rev 65:1357–1369

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