Complete assignment of Ala, Ile, Leu, Met and Val methyl groups of human blood group A and B glycosyltransferases using lanthanide-induced pseudocontact shifts and methyl

Complete assignment of Ala, Ile, Leu, Met and Val methyl groups of human blood group A and B glycosyltransferases using lanthanide-induced pseudocontact shifts and methyl–methyl NOESY

Published:2018-04 Issue:4 Volume:70 Page:245-259
ISSN:0925-2738
Container-title:Journal of Biomolecular NMR
language:en
Short-container-title:J Biomol NMR

Author:

Flügge Friedemann,Peters Thomas^ORCID

Funder

Deutsche Forschungsgemeinschaft

inext

Publisher

Springer Science and Business Media LLC

Subject

Spectroscopy,Biochemistry

Link

http://link.springer.com/article/10.1007/s10858-018-0183-4/fulltext.html

Reference51 articles.

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2. Amero C, Asunción Durá M, Noirclerc-Savoye M et al (2011) A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies. J Biomol NMR 50:229–236. https://doi.org/10.1007/s10858-011-9513-5

3. Atreya HS, Chary KVR (2001) Selective “unlabeling” of amino acids in fractionally 13C labeled proteins: an approach for stereospecific NMR assignments of CH3 groups in Val and Leu residues. J Biomol NMR 19:267–272. https://doi.org/10.1023/A:1011262916235

4. Ayala I, Sounier R, Usé N et al (2009) An efficient protocol for the complete incorporation of methyl-protonated alanine in perdeuterated protein. J Biomol NMR 43:111–119. https://doi.org/10.1007/s10858-008-9294-7

5. Chao F-A, Kim J, Xia Y et al (2014) FLAMEnGO 2.0: an enhanced fuzzy logic algorithm for structure-based assignment of methyl group resonances. J Magn Reson 245:17–23. https://doi.org/10.1016/j.jmr.2014.04.012

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