Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective 15N-labelling and production of perdeuterated proteins in H2O
Author:
Publisher
Springer Science and Business Media LLC
Subject
Spectroscopy,Biochemistry
Link
http://link.springer.com/content/pdf/10.1007/s10858-011-9477-5.pdf
Reference32 articles.
1. Apponyi MA, Ozawa K, Dixon NE, Otting G (2008) Cell-free protein synthesis for analysis by NMR spectroscopy. In: Kobe B, Guss M, Huber T (eds) Methods in molecular biology 426, structural proteomics: high-throughput methods. Humana Press, Totowa, pp 257–268
2. Berg JM, Tymoczko JL, Stryer L (2006) Biochemistry. WH Freeman and Company, New York
3. Eliot AC, Kirsch JF (2004) Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations. Annu Rev Biochem 73:383–4515
4. Etezady-Esfarjani T, Hiller S, Villalba C, Wüthrich K (2007) Cell-free synthesis of perdeuterated proteins for NMR studies. J Biomol NMR 39:229–238
5. Guignard L, Ozawa K, Pursglove SE, Otting G, Dixon NE (2002) NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach. FEBS Lett 524:159–162
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