A simple DNA recombination screening method by RT-PCR as an alternative to Southern blot-Reference-Cited by-同舟云学术

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A simple DNA recombination screening method by RT-PCR as an alternative to Southern blot

Published:2017-01-19 Issue:3 Volume:26 Page:429-434
ISSN:0962-8819
Container-title:Transgenic Research
language:en
Short-container-title:Transgenic Res

Author:

Albers Eliene,Sbroggiò Mauro,Martin-Gonzalez Javier,Avram Alexandra,Munk Stephanie,Lopez-Contreras Andres J.

Funder

European Research Council

Publisher

Springer Science and Business Media LLC

Subject

Agronomy and Crop Science,Genetics,Animal Science and Zoology,Biotechnology

Link

http://link.springer.com/article/10.1007/s11248-017-0008-3/fulltext.html

Reference9 articles.

1. Austin CP et al (2004) The knockout mouse project. Nat Genet 36(9):921–924

2. Capecchi MR (2005) Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat Rev Genet 6(6):507–512

3. Doudna JA, Charpentier E (2014) Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science 346(6213):1258096

4. Hall B, Limaye A, Kulkarni AB (2009) Overview: generation of gene knockout mice. Curr Protoc Cell Biol. Chapter 19: p. Unit 19 12 19 12 1-17

5. Inui M et al (2014) Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system. Sci Rep 4:5396

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