Abstract
AbstractA 3D-printed stereolithographic platform for selective biorecognition is designed to enable convective microscale affinity extraction of microcystin-LR (MC-LR) followed by direct solid-phase optosensing exploiting ratiometric front-face fluorescence spectroscopy. For this purpose, a recombinant monoclonal plantibody (recAb) is covalently attached to a 3D-printed structure for sorptive immunoextraction, whereupon the free and unbound primary amino moieties of the recAb are derivatized with a fluorescent probe. The fluorophore-recAb-MC-LR laden device is then accommodated in the cuvette holder of a conventional fluorometer without any instrumental modification for the recording of the solid-phase fluorescence emission. Using Rodbard’s four-parameter sigmoidal function, the 3D-printed bioselective platform features a limit of detection (LOD) of 28 ng L−1 using a sample volume of 500 mL, device-to-device reproducibility down to 12%, and relative recoveries ranging from 91 to 100% in marine waters. Printed prototypes are affordable, just 0.4 € per print and ≤ 10 € per device containing recAb. One of the main assets of the miniaturized immunoextraction device is that it performs comparably well in terms of analytical figures of merit with costly mass spectrometric-based analytical methodologies, such as HPLC–MS/MS. The device is readily applicable to high-matrix samples, such as seawater, as opposed to previous biosensing platforms, just applied to freshwater systems.
Graphical abstract
Funder
Ministerio de Ciencia, Innovación y Universidades
Conselleria de Cultura, Educación y Ciencia, Generalitat Valenciana
Universitat de Valencia
Publisher
Springer Science and Business Media LLC
Reference31 articles.
1. Catherine A, Bernard C, Spoof L, Bruno M (2016) Microcystins and nodularins, In: Meriluoto J, Spoof L and Codd GA (eds) Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis. John Wiley & Sons, Ltd, p 107–126. https://doi.org/10.1002/9781119068761.ch11
2. Mowe MAD, Mitrovic SM, Lim RP, Furey A, Yeo DCJ (2015) Tropical cyanobacterial blooms: a review of prevalence, problem taxa, toxins and influencing environmental factors. J Limnol 74. https://doi.org/10.4081/jlimnol.2014.1005
3. MacKintosh C, Beattie KA, Klumpp S, Cohen P, Codd GA (1990) Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and higher plants. FEBS Lett 264:187–192. https://doi.org/10.1016/0014-5793(90)80245-e
4. Massey IY, Yang F, Ding Z, Yang S, Guo J, Tezi C, Al-Osman M, Kamegni RB, Zeng W (2018) Exposure routes and health effects of microcystins on animals and humans: A mini-review. Toxicon 151:156–162. https://doi.org/10.1016/j.toxicon.2018.07.010
5. World Health Organization (WHO) (2003) Cyanobacterial toxins: microcystin-LR in drinking-water. Background document for development of WHO guidelines for drinking-water quality, health criteria and other supporting information. WHO/SDE/WSH/03.04/57, Geneva www.who.int/docs/default-source/wash-documents/wash-chemicals/cyanobacterial-toxins-background-document.pdf?sfvrsn=46de6339_4. Accessed 21/05/2024