Adaptation of cholesterol-requiring NS0 mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-free culture medium

Author:

Keen M. J.,Steward T. W.

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Clinical Biochemistry,Biomedical Engineering,Bioengineering,Biotechnology

Reference16 articles.

1. Bebbington CR, Renner G, Thomson S, King D, Abrams D and Yarranton GT (1992) High-level expression of recombinant antibody from myeloma cells using glutamine synthetase as an amplifiable selectable marker. Bio/technology 10: 169175.

2. Chen JK and Li L (1987) Sterol depletion reduces receptor-mediated low-density lipoprotein binding in NS-1 mouse myeloma cells. Exp. Cell Res. 171: 76?85.

3. Darffer FJ (1990) A protein-free medium for the growth of hybridomas and other cells of the immune system. In Vitro Cell Dev. Biol. 26: 769?778.

4. Galfre G and Milstein C (1982) Chemical typing of human kappa light chain subgroups expressed by human hybrid myelomas. Immunology 46: 125?127.

5. Galfre G and Milstein C (1981) Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymmol 73B: 1?46.

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