Site-directed mutagenesis by double polymerase chain reaction

Author:

Barik Sailen

Publisher

Springer Science and Business Media LLC

Subject

Molecular Biology,Applied Microbiology and Biotechnology,Biochemistry,Bioengineering,Biotechnology

Reference9 articles.

1. Sarkar, G. and Sommer, S. S. (1990) The “megaprimer” method of site-directed mutagenesis.BioTechniques 8, 404–407.

2. Barik, S. and Galinski, M. (1991) “Megaprimer” method of PCR: Increased template concentration improves yield.BioTechniques 10, 489–490.

3. Barik, S. (1995) Site-directed mutagenesis by PCR; substitution, insertion, deletion, and gene fusion.Methods Neurosci.,26, 309–323.

4. Clark, J. M. (1988) Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.Nucleic Acids Res.16, 9677–9686.

5. Maniatis, T., Fritsch, E. F., and Sambrook, J., (1982)Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 113–119.

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