How to Deal with PCR Contamination in Molecular Microbial Ecology
Author:
Publisher
Springer Science and Business Media LLC
Subject
Soil Science,Ecology,Ecology, Evolution, Behavior and Systematics
Link
http://link.springer.com/content/pdf/10.1007/s00248-014-0453-y.pdf
Reference13 articles.
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2. Van Dongen WFD, White J, Brandl HB et al (2013) Age-related differences in the cloacal microbiota of a wild bird species. BMC Ecol 13:11. doi: 10.1186/1472-6785-13-11
3. Mühl H, Kochem A-J, Disqué C, Sakka SG (2010) Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood. Diagn Microbiol Infect Dis 66:41–9. doi: 10.1016/j.diagmicrobio.2008.07.011
4. Spangler R, Goddard NL, Thaler DS (2009) Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria. PLoS One 4:e7010. doi: 10.1371/journal.pone.0007010
5. Borst A, Box AT, Fluit AC (2004) False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy. Eur J Clin Microbiol Infect Dis 23:289–99. doi: 10.1007/s10096-004-1100-1
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