Abstract
Abstract
Purpose
We report the manufacture of particles containing a mixture of hydroxyapatite–argentum–titanium oxide (HAT), followed by attachment to nonwoven polyester fabrics to produce HAT-coated sheets (HATS) for use in masks. The purpose of the present study was to perform cellular, in vivo, and clinical studies to further examine the safety of HATS for use in masks to improve nasal allergy.
Methods
Reverse mutation tests for HAT were performed using five bacterial strains. A cellular toxicity test was performed using a Chinese hamster cell line incubated with the HATS extracts. Skin reactions after intradermal administration were examined in rabbits. Skin sensitization tests in guinea pigs were performed using the HATS extracts. HAT was administered to the nasal cavity and conjunctival sac of the rabbits. An oral administration study was performed in rats. Finally, a human skin patch test was performed using the HATS.
Results
Reverse mutation tests showed negative results. The cellular toxicity test showed that the HATS extract had moderate cytotoxicity. The intradermal skin reaction and skin sensitization tests were all negative. The administration of HAT to the nasal cavity and intraocular administration showed negative results. No toxicity was observed after oral administration of HAT powder up to a dose of 2000 mg/kg. Finally, the skin patch test result was negative.
Conclusion
Although HAT showed moderate cytotoxicity, in vivo results indicated that HAT is safe because it does not come in direct contact with cells in normal usage, and HATS is safe when used in masks.
Funder
DR.C Medical Medicine Co., Ltd.
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Otorhinolaryngology
Reference36 articles.
1. Okazaki N, Yamaki D, Takei T, Shimizu M, Kamatani N, Shindo TB (2022) Studies on safety and efficacy of particles containing a mixture of hydroxyapatite–argentum–titanium oxide (HAT) and sheets coated with HAT particles to be used in masks to improve nasal allergy: I. in vitro studies. Allergy Pract (Tokyo) 42:203–212
2. Coronado JM, Fresno F, Hernández-Alonso MD et al (2013) Design of advanced photocatalytic materials for energy and environmental applications. Springer, London, pp 1–5
3. Nozik AJ (1977) Photochemical diodes. Appl Phys Lett 30:567–569
4. Yao Y, Ohko Y, Sekiguchi Y et al (2008) Self-sterilization using silicone catheters coated with Ag and TiO2 nanocomposite thin film. J Biomed Mater Res B Appl Biomater 85:453–460. https://doi.org/10.1111/j.1442-2042.2007.01743.x
5. Ohko Y, Tatsuma T, Fujii T et al (2003) Multicolor photochromism of TiO films loaded with Ag nanoparticles. Nat Mater 2:29–31. https://doi.org/10.1038/nmat796
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