Antibodies to guanylic acid: Fractionation and specificities

Abstract

Guanylic-acid-specific antibodies were elicited in rabbits) using as immunogen pG linked through 5′-phosphate to thyroglobulin. Specificity and affinity of antibodies to nucleotides) nucleosides, DNA, and RNA were studied by their binding to radioactive ligands and competition experiments. Guanylic-acid-specific antibodies do not bind to deoxyguanylic acid and have an average association constant of 107 M−1 at 4°C Binding of the antibodies to3H-RNA is G-specifiC. The antibodies do not bind to32P-ssDNA or32P-dsDNA. The pG-specific antibodies could be separated into different fractions by affinity chromatography. These fractions) though specific to pG) differ in their cross-reactivities to nucleosides and nucleotides.