The Downregulation of MMP23B Facilitates the Suppression of Vitality and Induction of Apoptosis in Endometrial Cancer Cells

Author:

Li NingORCID,Li HuaORCID,Wei LijuanORCID,Chen HuiORCID,Wu ZhaorongORCID,Yuwen SiORCID,Yang SufangORCID

Abstract

AbstractEndometrial cancer is a malignant tumor that commonly occurs in the female reproductive system and its incidence is still increasing. The mechanism of the development of endometrial cancer has not yet been fully clarified, so we need to continuously study the relevant mechanisms of endometrial cancer and continue to explore its biomarkers in order to discover more precise and effective treatment methods for endometrial cancer. RT-qPCR (Real-Time quantitative Polymerase Chain Reaction) experiments were used to detect the expression level of MMP23B (Matrix Metalloproteinase 23B) in endometrial cancer cells; the clinical data of the TCGA (The Cancer Genome Atlas) database were downloaded, and gene expression profiles were analyzed to investigate the correlation between MMP23B (Matrix Metalloproteinase 23B) and the survival prognosis of endometrial cancer, and functional enrichment analysis was performed on MMP23B (Matrix Metalloproteinase 23B) related genes. After silencing MMP23B (Matrix Metalloproteinase 23B), CCK8 (Cell Counting Kit-8), RT-qPCR (Real-Time quantitative Polymerase Chain Reaction), scratch assay, and transwell assay were used to detect cell viability, levels of apoptotic factors, migration rate, and invasion number of endometrial cancer, respectively. MMP23B (Matrix Metalloproteinase 23B) was highly expressed in endometrial cancer, which is closely related to a poor survival prognosis for endometrial cancer, and may act on endometrial cancer through apoptosis-related functions. The downregulation of MMP23B (Matrix Metalloproteinase 23B) reduced the cell viability of endometrial cancer cells, upregulated the expression levels of CASP3 (Caspase-3), CASP8 (Caspase-8) and CASP9 (Caspase-9) in cells, and inhibited cell migration and invasion.

Funder

2023 Traditional Chinese Medicine Appropriate Technology Development and Promotion Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine

Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine

2020 PhD Introduction Research Initiation Fund Project of Guangxi University of Traditional Chinese Medicine

2021 Talent Introduction Research Initiation Fund Project of Guangxi International Zhuang Medicine Hospital

National Natural Science Foundation of China

Natural Science Foundation of Guangxi Province

Publisher

Springer Science and Business Media LLC

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