Abstract
AbstractHydrolysis of cellulose in concentrated phosphoric acid is known to give two distinctive mono-disperse cello-oligomers: the degree of polymerization (DP) of 7 and 15. To understand the formation mechanism of monodisperse cello-oligomers, the transition of DP during phosphoric hydrolysis was monitored by size-exclusion chromatography combined with multiangle laser-light scattering analyses (SEC/MALLS). The obtained results suggested that not the hydrolysis at a specific length but random hydrolysis was likely. The rate of hydrolysis slowed down when the DP reached around 40, and the pool of cello-oligomers appeared at the incubation of 35 days. Then, the mono-disperse cello-oligomers were recovered from the pool of cello-oligomers by fractionation based on the solubility difference.
Funder
New Energy and Industrial Technology Development Organization
Japan Society for the Promotion of Science
Institut Carnot PolyNat
Publisher
Springer Science and Business Media LLC