Author:
Chaikam Vijay,Lopez Luis Antonio,Martinez Leocadio,Burgueño Juan,Boddupalli Prasanna M.
Abstract
AbstractIn vivo haploid induction in high frequency followed by efficient identification of haploids are important components of deriving completely homozygous doubled haploid (DH) lines in maize. Several genetic marker systems were proposed and/or used for identification of in vivo maternal haploids in maize, such as R1-nj (Navajo), high oil, red root and transgenic markers. In this study, we propose a new method of haploid/diploid identification based on natural differences in seedling traits of haploids and diploids, which can be used in any induction cross independently of the genetic marker systems. Using confirmed haploids and diploids from five different populations, the study established that haploid and diploid seedlings exhibit significant differences for seedling traits, particularly radicle length (RL), coleoptile length (CL), and number of lateral seminal roots (NLSR). In six populations that exhibited complete inhibition of the commonly used R1-nj (Navajo) marker, we could effectively differentiate haploids from diploids by visual inspection of the seedling traits. In the haploid seed fraction identified based on R1-nj marker in ten populations, false positives were reduced several-fold by early identification of haploids at seedling stage using the seedling traits. We propose that seedling traits may be integrated at the haploid identification stage, especially in populations that are not amenable to use of genetic markers, and for improving the efficiency of DH line production by reducing the false positives.
Funder
SAGARPA
CGIAR Research Program MAIZE
Bill and Melinda Gates Foundation
Publisher
Springer Science and Business Media LLC
Subject
Horticulture,Plant Science,Genetics,Agronomy and Crop Science
Reference27 articles.
1. Chaikam V, Mahuku G (2012) Chromosome doubling of maternal haploids. In: Prasanna BM, Chaikam V, Mahuku G (eds) Doubled haploid technology in maize breeding: theory and practice. CIMMYT, Mexico, pp 24–29
2. Chaikam V, Prasanna BM (2012) Maternal haploid detection using anthocyanin markers. In: Prasanna BM, Chaikam V, Mahuku G (eds) Doubled haploid technology in maize breeding: theory and practice. CIMMYT, Mexico, pp 20–23
3. Chaikam V, Mahuku G, Prasanna BM (2012) Design and implementation of maternal haploid induction. In: Prasanna BM, Chaikam V, Mahuku G (eds) Doubled haploid technoogy in maize breeding: theory and practice. CIMMYT, Mexico, pp 14–19
4. Chaikam V, Nair SK, Babu R et al (2015) Analysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression. Theor Appl Genet 128:159–171
5. Chaikam V, Martinez L, Melchinger AE, Schipprack W, Prasanna BM (2016) Development and validation of red root marker-based haploid inducers in Maize. Crop Sci 56:1678–1688
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