Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells-Reference-Cited by-同舟云学术

Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells

Published:2023-01-04 Issue:4 Volume:159 Page:353-361
ISSN:0948-6143
Container-title:Histochemistry and Cell Biology
language:en
Short-container-title:Histochem Cell Biol

Author:

Atiakshin Dmitri,Kostin Andrey,Buchwalow Igor^ORCID,Morozow Denis,Samoilova Vera,Tiemann Markus

Abstract

AbstractThis study provides a combined histochemical method for detecting enzyme activity of chloroacetate esterase simultaneously with immunolabeling of the components of a specific tissue microenvironment on formalin-fixed, paraffin-embedded specimens. Chromogenic detection of the molecular targets within and outside the mast cells provides novel options in determining the histoarchitectonics of organ-specific mast cell populations, studying the functional significance of chloroacetate esterase and specifying the immune landscape of the tissue microenvironment.

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Medical Laboratory Technology,Molecular Biology,Histology

Link

https://link.springer.com/content/pdf/10.1007/s00418-022-02174-1.pdf

Reference33 articles.

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2. Atiakshin D, Buchwalow I, Samoilova V, Tiemann M (2018) Tryptase as a polyfunctional component of mast cells. Histochem Cell Biol 149(5):461–477. https://doi.org/10.1007/s00418-018-1659-8

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4. Atiakshin DA, Shishkina VV, Gerasimova OA, Meshkova VY, Samodurova NY, Samoilenko TV, Buchwalow IB, Samoilova VE, Tiemann M (2021) Combined histochemical approach in assessing tryptase expression in the mast cell population. Acta Histochem 123(4):151711. https://doi.org/10.1016/j.acthis.2021.151711

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