Abstract
AbstractFluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and applications in the relatively understudied area of multiscale fluorescence imaging of living samples. We discuss fundamental challenges in live multiscale imaging and describe successful examples that highlight the power of this approach. We attempt to synthesize general strategies from these test cases, aiming to help accelerate progress in this exciting area.
Funder
Intramural research program of the National Institute of Biomedical Imaging and Bioengineering within the National Institutes of Health.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Medical Laboratory Technology,Molecular Biology,Histology
Cited by
6 articles.
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