Klebsiella pneumoniae carrying multiple alleles of antigen 43-encoding gene of Escherichia coli associated with biofilm formation-Reference-Cited by-同舟云学术

Klebsiella pneumoniae carrying multiple alleles of antigen 43-encoding gene of Escherichia coli associated with biofilm formation

Published:2023-01-25 Issue:3 Volume:42 Page:371-377
ISSN:0934-9723
Container-title:European Journal of Clinical Microbiology & Infectious Diseases
language:en
Short-container-title:Eur J Clin Microbiol Infect Dis

Author:

Tambassi Martina^ORCID,Passarini Elena,Menozzi Ilaria^ORCID,Berni Melissa^ORCID,Bracchi Chiara^ORCID,Dodi Alessandra^ORCID,Bolzoni Luca^ORCID,Scaltriti Erika^ORCID,Morganti Marina^ORCID,Ferrarini Giulia,Sordi Laura,Sarti Mario,Ambretti Simone^ORCID,Pongolini Stefano^ORCID

Abstract

AbstractA clinical strain of Klebsiella pneumoniae typed as sequence type 307 carrying three different alleles of the flu gene encoding the Escherichia coli virulence factor antigen 43 associated with biofilm formation was detected and characterized. The flu alleles are located in the chromosome inside putative integrative conjugative elements. The strain displays the phenotypes associated with Ag43, i.e. bi-phasic colony morphology and enhanced biofilm production. Furthermore, the strain produces low amount of capsule known to affect Ag43 function. Analysis of 1431 worldwide deposited genomes revealed that 3.7% Klebsiella pneumoniae carry one or two flu alleles.

Publisher

Springer Science and Business Media LLC

Subject

Infectious Diseases,Microbiology (medical),General Medicine

Link

https://link.springer.com/content/pdf/10.1007/s10096-023-04552-6.pdf

Reference35 articles.

1. Holt KE, Wertheim H, Zadoks RN, Baker S, Whitehouse CA, Dance D, Jenneyb A, Connork TR, Yang Hsum L, Severinn J, Brisseo S, Caob H, Wilkschb J, Gorriea C, Schultza MB, Edwardsa DJ, Van Nguyenq K, Vu Nguyenq T, Tuyet Daoq T, Mensinke M, Le Minhg V, Thi Khanh Nhug N, Schultszg C, Kuntamanu K, Newtond PN, Moored CE, Strugnellb RA, Thomson NR (2015) Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health. Proc Natl Acad Sci USA 112:E3574–E3581. https://doi.org/10.1073/pnas.1501049112

2. Paczosa MK, Mecsas J (2016) Klebsiella pneumoniae: going on the offense with a strong defense. Microbiol Mol Biol Rev 80:629–661. https://doi.org/10.1128/MMBR.00078-15

3. Bowler P, Murphy C, Wolcott R (2020) Biofilm exacerbates antibiotic resistance: is this a current oversight in antimicrobial stewardship? Antimicrob Resist Infect Control 9:162. https://doi.org/10.1186/s13756-020-00830-6

4. Owen P, Meehan M, de Loughry-Doherty H, Henderson I (1996) Phase-variable outer membrane proteins in Escherichia coli. FEMS Immunol Med Microbiol 16:63–76. https://doi.org/10.1111/j.1574-695X.1996.tb00124.x

5. Henderson IR, Meehan M, Owen P (1997) Antigen 43, a phase-variable bipartite outer membrane protein, determines colony morphology and autoaggregation in Escherichia coli K-12. FEMS Microbiol Lett 149:115–120. https://doi.org/10.1111/j.1574-6968.1997.tb10317.x