Rapid and simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes by magnetic capture hybridization and multiplex real-time PCR

Author:

Carloni Elisa,Rotundo Luca,Brandi Giorgio,Amagliani GiuliaORCID

Publisher

Springer Science and Business Media LLC

Subject

General Medicine,Microbiology

Reference29 articles.

1. Algaba IG, Geerts M, Jennes M, Coucke W, Opsteegh M, Cox E, Dorny P, Dierick K, De Craeye S (2017) A more sensitive, efficient and ISO 17025 validated magnetic capture real time PCR method for the detection of the archetypal Toxoplasma gondii strains in meat. Int J Parasitol doi: https://doi.org/10.1016/j.ijpara.2017.05.005

2. Amagliani G, Omiccioli E, Campo A, Bruce IJ, Brandi G, Magnani M (2006) Development of a magnetic capture hybridization-PCR assay for Listeria monocytogenes direct detection in milk samples. J Appl Microbiol 100:375–383. https://doi.org/10.1111/j.1365-2672.2005.02761.x

3. Amagliani G, Omiccioli E, Brandi G, Bruce IJ, Magnani M (2010) A multiplex magnetic capture hybridisation and multiplex real-time PCR protocol for pathogen detection in seafood. Food Microbiol 27:580–585. https://doi.org/10.1016/j.fm.2010.01.007

4. Amagliani G, Petruzzelli A, Carloni E, Tonucci F, Foglini M, Micci E, Ricci M, Di Lullo S, Rotundo L, Brandi G (2016) Presence of Escherichia coli O157, Salmonella spp., and Listeria monocytogenes in raw ovine milk destined for cheese production and evaluation of the equivalence between the analytical methods applied. Foodborne Pathog Dis 13:626–632. https://doi.org/10.1089/fpd.2016.2159

5. Brusa V, Galli L, Linares LH, Ortega EE, Lirón JP, Leotta GA (2015) Development and validation of two SYBR green PCR assays and a multiplex real-time PCR for the detection of Shiga toxin-producing Escherichia coli in meat. J Microbiol Methods 119:10–17. https://doi.org/10.1016/j.mimet.2015.09.013

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