Initial Effect of Temperature Rise on α-Synuclein Aggregation – Entropic Forces Drive the Exposure of Protein Hydrophobic Groups Probed by Fluorescence Spectroscopy
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Published:2023-02-24
Issue:5
Volume:33
Page:1727-1738
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ISSN:1053-0509
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Container-title:Journal of Fluorescence
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language:en
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Short-container-title:J Fluoresc
Author:
Saraiva Marco A.,Florêncio M. Helena
Abstract
AbstractThe aberrant formation of α-synuclein (Syn) aggregates, varying in size, structure and morphology, has been linked to the development of Parkinson’s disease. In the early stages of Syn aggregation, large protein amyloid aggregates with sizes > 100 nm in hydrodynamic radius have been noticed. These low overall abundant large Syn aggregates are notoriously difficult to study by conventional biophysical methods. Due to the growing importance of studying the early stages of Syn aggregation, we developed a strategy to achieve this purpose, which is the study of the initial effect of the Syn protein aqueous solutions temperature rise. Therefore, the increase of the Syn aqueous solutions entropy by the initial effect of the temperature rise led to the exposure of the protein hydrophobic tyrosyl groups by not interfering with this amyloid protein aggregation. As an attempt to interpret the degree of the referred protein tyrosyl groups exposure, the classic rotameric conformations of the Nα-acetyl-L-tyrosinamide (NAYA) parent compound were used. For both NAYA and Syn, it was determined that the classic rotameric conformations involving the tyrosyl groups indeed accounted for their exposure under steady-state conditions of fluorescence, for lowest molecular species concentrations investigated at least. In this situation, Syn aggregation was observed. For the higher NAYA and Syn concentrations studied, the referred classic rotameric conformation were insufficient in such referred steady-state conditions and, for Syn, in particular, fluorescence anisotropy measurements revealed that less protein aggregation occurs along with its delay. Overall, the developed strategy by focusing on the initial effect of the temperature rise of Syn aqueous solutions in lower concentrations is suitable for informing us about the degree of this protein aggregation in solution.
Funder
Fundação para a Ciência e a Tecnologia Portuguese Mass Spectrometry Network Universidade de Lisboa
Publisher
Springer Science and Business Media LLC
Subject
Law,Clinical Biochemistry,Spectroscopy,Sociology and Political Science,Social Sciences (miscellaneous),Clinical Psychology,Biochemistry
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