Abstract
AbstractSevere acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) amplicon contamination was discovered due to next-generation sequencing (NGS) reads mapping in the negative controls. Environmental screening was undertaken to determine the source of contamination, which was suspected to be evaporation during polymerase chain reaction (PCR) assays while using the coronavirus disease 2019 (COVID-19) ARTIC protocol. A decontamination strategy is hereby documented to assist laboratories that may experience similar amplicon contamination. Routine molecular laboratory environmental screening as a quality control is highly recommended.
Funder
Bill and Melinda Gates Foundation
World Health Organization
National Research Foundation
Poliomyelitis Research Foundation
Publisher
Springer Science and Business Media LLC
Subject
Virology,General Medicine
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