Rapid differential detection of subtype H1 and H3 swine influenza viruses using a TaqMan-MGB-based duplex one-step real-time RT-PCR assay

Author:

Chen Kaibiao,Kong Ming,Liu Jiao,Jiao Jun,Zeng Zixiong,Shi Liwei,Bu Xinxin,Yan Yayao,Chen Yu,Gao Ruyi,Liu Xiaowen,Wang Xiaoquan,Hu Jiao,Hu Shunlin,Jiao Xinan,Liu Xiufan,Gu MinORCID

Funder

National Key Research and Development Project of China

Jiangsu Provincial Natural Science Fund for Excellent Young Scholars

Special Financial Grant from the China Postdoctoral Science Foundation

Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Jiangsu Qinglan Project, the High-end talent support program of Yangzhou University

Publisher

Springer Science and Business Media LLC

Subject

Virology,General Medicine

Reference27 articles.

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2. Ruan BY, Wen F, Gong XQ, Liu XM, Wang Q, Yu LX, Wang SY, Zhang P, Yang HM, Shan TL, Zheng H, Zhou YJ, Tong W, Gao F, Tong GZ, Yu H (2018) Protective efficacy of a high-growth reassortant H1N1 influenza virus vaccine against the European Avian-like H1N1 swine influenza virus in mice and pigs. Vet Microbiol 222:75–84. https://doi.org/10.1016/j.vetmic.2018.07.003

3. Van Reeth K, Nauwynck H, Pensaert M (1996) Dual infections of feeder pigs with porcine reproductive and respiratory syndrome virus followed by porcine respiratory coronavirus or swine influenza virus: a clinical and virological study. Vet Microbiol 48(3–4):325–335. https://doi.org/10.1016/0378-1135(95)00145-x

4. Van Reeth K, Nauwynck H, Pensaert M (2001) Clinical effects of experimental dual infections with porcine reproductive and respiratory syndrome virus followed by swine influenza virus in conventional and colostrum-deprived pigs. J Vet Med B Infect Dis Vet Public Health 48(4):283–292. https://doi.org/10.1046/j.1439-0450.2001.00438.x

5. Lewis NS, Russell CA, Langat P, Anderson TK, Berger K, Bielejec F, Burke DF, Dudas G, Fonville JM, Fouchier RA, Kellam P, Koel BF, Lemey P, Nguyen T, Nuansrichy B, Peiris JM, Saito T, Simon G, Skepner E, Takemae N, Webby RJ, Van Reeth K, Brookes SM, Larsen L, Watson SJ, Brown IH, Vincent AL (2016) The global antigenic diversity of swine influenza A viruses. Elife 5:e12217. https://doi.org/10.7554/eLife.12217

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