A fused hybrid enzyme of 8-hydroxygeraniol oxidoreductase (8HGO) from Gardenia jasminoides and iridoid synthase (ISY) from Catharanthus roseus significantly enhances nepetalactol and iridoid production
Author:
Funder
the Guangdong Provincial Rural Revitalization Strategy
Guangdong Provincial Pearl River Talents Program
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Genetics
Link
https://link.springer.com/content/pdf/10.1007/s00425-023-04287-w.pdf
Reference38 articles.
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2. Awadasseid ALW, Liu Z, Qiao C, Pang J, Zhang G, Luo Y (2020) Characterization of Camptotheca acuminata 10-hydroxygeraniol oxidoreductase and iridoid synthase and their application in biological preparation of nepetalactol in Escherichia coli featuring NADP(+)-NADPH cofactors recycling. Int J Biol MaCromol 162:1076–1085
3. Billingsley JM, DeNicola AB, Barber JS, Tang MC, Horecka J, Chu A, Garg NK, Tang Y (2017) Engineering the biocatalytic selectivity of iridoid production in Saccharomyces cerevisiae. Metab Eng 44:117–125. https://doi.org/10.1016/j.ymben.2017.09.006
4. Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ (2016) Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids. ACS Synth Biol 5(5):405–414. https://doi.org/10.1021/acssynbio.5b00289
5. Chen LP, Li MX, Yang ZQ, Tao WD, Wang P, Tian XY, Li XL, Wang WG (2020) Gardenia jasminoides Ellis: Ethnopharmacology, phytochemistry, and pharmacological and industrial applications of an important traditional Chinese medicine. J Ethnopharmacol 257:28. https://doi.org/10.1016/j.jep.2020.112829
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