Purification and characterization of recombinantStreptomyces clavuligerus isopenicillin N synthase produced inEscherichia coli
Author:
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,Applied Microbiology and Biotechnology,Biotechnology,Bioengineering
Link
http://link.springer.com/content/pdf/10.1007/BF01570004.pdf
Reference21 articles.
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2. Baldwin JE, JM Blackburn, JD Sutherland and MC Wright. 1991. High-level soluble expression of isopenicillin N synthase isozymes inE. coli. Tetrahedron 47: 5991–6002.
3. Blackshear PJ. 1984. Systems for polyacrylamide gel electrophoresis. Methods Enzymol 104: 237–255.
4. Bradford M. 1976. A rapid, sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem 72: 248–254.
5. Chalmers JJ, E Kim, JN Telford, EY Wong, WC Tacon, ML Shuler and DB Wilson. 1990. Effects of temperature onEscherichia coli over-producing β-lactamase or human growth factor. Appl Environ Microbiol 56: 104–111.
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