Induced responses to the wheat pathogen: Tan Spot—(Pyrenophora tritici-repentis) in wheat (Triticum aestivum) focus on changes in defence associated and sugar metabolism
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Published:2024-01-31
Issue:1
Volume:20
Page:
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ISSN:1573-3890
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Container-title:Metabolomics
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language:en
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Short-container-title:Metabolomics
Author:
Ferreira Larissa CarvalhoORCID, Santana Flavio MartinsORCID, Scagliusi Sandra Maria MansurORCID, Beckmann ManfredORCID, Mur Luis A. J.ORCID
Abstract
Abstract
Introduction
Tan Spot (TS) disease of wheat is caused by Pyrenophora tritici-repentis (Ptr), where most of the yield loss is linked to diseased flag leaves. As there are no fully resistant cultivars available, elucidating the responses of wheat to Ptr could inform the derivation of new resistant genotypes.
Objectives
The study aimed to characterise the flag-leaf metabolomes of two spring wheat cultivars (Triticum aestivum L. cv. PF 080719 [PF] and cv. Fundacep Horizonte [FH]) following challenge with Ptr to gain insights into TS disease development.
Methods
PF and FH plants were inoculated with a Ptr strain that produces the necrotrophic toxin ToxA. The metabolic changes in flag leaves following challenge (24, 48, 72, and 96 h post-inoculation [hpi]) with Ptr were investigated using untargeted flow infusion ionisation-high resolution mass spectroscopy (FIE-HRMS).
Results
Both cultivars were susceptible to Ptr at the flag-leaf stage. Comparisons of Ptr- and mock-inoculated plants indicated that a major metabolic shift occurred at 24 hpi in FH, and at 48 hpi in PF. Although most altered metabolites were genotype specific, they were linked to common pathways; phenylpropanoid and flavonoid metabolism. Alterations in sugar metabolism as well as in glycolysis and glucogenesis pathways were also observed. Pathway enrichment analysis suggested that Ptr-triggered alterations in chloroplast and photosynthetic machinery in both cultivars, especially in FH at 96 hpi. In a wheat-Ptr interactome in integrative network analysis, “flavone and flavonol biosynthesis” and “starch and sucrose metabolism” were targeted as the key metabolic processes underlying PF–FH–Ptr interactions.
Conclusion
These observations suggest the potential importance of flavone and flavonol biosynthesis as well as bioenergetic shifts in susceptibility to Ptr. This work highlights the value of metabolomic approaches to provide novel insights into wheat pathosystems.
Funder
Aberystwyth University
Publisher
Springer Science and Business Media LLC
Subject
Clinical Biochemistry,Biochemistry,Endocrinology, Diabetes and Metabolism
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