The relative abundance of fecal bacterial species belonging to the Firmicutes and Bacteroidetes phyla is related to plasma levels of bile acids in young adults
-
Published:2023-06-06
Issue:6
Volume:19
Page:
-
ISSN:1573-3890
-
Container-title:Metabolomics
-
language:en
-
Short-container-title:Metabolomics
Author:
Osuna-Prieto Francisco J.ORCID, Xu HuiwenORCID, Ortiz-Alvarez LourdesORCID, Di Xinyu, Kohler IsabelleORCID, Jurado-Fasoli LucasORCID, Rubio-Lopez JoseORCID, Plaza-Díaz JulioORCID, Vilchez-Vargas RamiroORCID, Link AlexanderORCID, Gil AngelORCID, Ruiz Jonatan R.ORCID, Rensen Patrick C. N.ORCID, Martinez-Tellez BorjaORCID
Abstract
Abstract
Background
Gut bacteria play a crucial role in the metabolism of bile acids (BA). Whether an association exists between the fecal microbiota composition and circulating BA levels in humans is poorly understood. Here, we investigated the relationship between fecal microbiota diversity and composition with plasma levels of BA in young adults.
Methods
Fecal microbiota diversity/composition was analyzed with 16S rRNA sequencing in 80 young adults (74% women; 21.9 ± 2.2 years old). Plasma levels of BA were measured using liquid chromatography-tandem mass spectrometry. PERMANOVA and Spearman correlation analyses were used to investigate the association between fecal microbiota parameters and plasma levels of BA.
Results
Fecal microbiota beta (P = 0.025) and alpha diversity indexes of evenness (rho = 0.237, P = 0.033), Shannon (rho = 0.313, P = 0.004), and inverse Simpson (rho = 0.283, P = 0.010) were positively associated with plasma levels of the secondary BA glycolithocholic acid (GLCA). The relative abundance of genera belonging to the Firmicutes and Bacteroidetes phyla was positively correlated with plasma levels of GLCA (all rho ≥ 0.225, P ≤ 0.049). However, the relative abundance of species from Firmicutes and Bacteroidetes phyla were negatively correlated with plasma levels of primary and secondary BA (all rho ≤ − 0.220, P ≤ 0.045), except for the relative abundance of Bacteroides vulgatus, Alistipes onderdonkii, and Bacteroides xylanisolvens species (Bacteroidetes phylum) that were positively correlated with the plasma levels of GLCA.
Conclusions
The relative abundance of specific fecal bacteria species is associated with plasma levels of BA in young adults. However, further investigations are required to validate whether the composition of the gut microbiota can regulate the plasma concentrations of BA in humans.
Funder
Spanish Ministry of Education Chinese Scholarship Council Spanish Ministry of Economy and Competitiveness via Fondo de Investigación Sanitaria del Instituto de Salud Carlos III Spanish Ministry of Economy and Competitiveness European Regional Development Funds University of Granada Plan Propio de Investigación 2016-Excellence actions the Junta de Andalucía, Consejería de Economía, Conocimiento, Empresas y Universidad AstraZeneca HealthCare Foundation Fundación Alfonso Martin Escudero Ministerio de Universidades Universidad de Granada
Publisher
Springer Science and Business Media LLC
Subject
Clinical Biochemistry,Biochemistry,Endocrinology, Diabetes and Metabolism
Reference43 articles.
1. Begley, M., Gahan, C. G. M., & Hill, C. (2005). The interaction between bacteria and bile. FEMS Microbiology Reviews. https://doi.org/10.1016/j.femsre.2004.09.003 2. Bonder, M. J., Kurilshikov, A., Tigchelaar, E. F., Mujagic, Z., Imhann, F., Vila, A. V., Deelen, P., Vatanen, T., Schirmer, M., Smeekens, S. P., Zhernakova, D. V., Jankipersadsing, S. A., Jaeger, M., Oosting, M., Cenit, M. C., Masclee, A. A., Swertz, M. A., Li, Y., & Kumar, V. (2016). The effect of host genetics on the gut microbiome. Nature Genetics, 48(11), 1407–1412. https://doi.org/10.1038/ng.3663 3. Cole, J. R., Wang, Q., Fish, J. A., Chai, B., McGarrell, D. M., Sun, Y., et al. (2014). Ribosomal Database Project: Data and tools for high throughput rRNA analysis. Nucleic Acids Research. https://doi.org/10.1093/nar/gkt1244 4. David, L. A., Maurice, C. F., Carmody, R. N., Gootenberg, D. B., Button, J. E., Wolfe, B. E., Ling, A. V., Devlin, A. S., Varma, Y., Fischbach, M. A., Biddinger, S. B., Dutton, R. J., & Dutton, R. J. (2014). Diet rapidly and reproducibly alters the human gut microbiome. Nature, 505(7484), 559–563. https://doi.org/10.1038/nature12820 5. De Smet, I., Van Hoorde, L., Vande Woestyne, M., Christiaens, H., & Verstraete, W. (1995). Significance of bile salt hydrolytic activities of lactobacilli. Journal of Applied Bacteriology, 79(3), 292–301. https://doi.org/10.1111/j.1365-2672.1995.tb03140.x
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|