Untargeted analysis of plasma samples from pre-eclamptic women reveals polar and apolar changes in the metabolome
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Published:2019-11-27
Issue:12
Volume:15
Page:
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ISSN:1573-3882
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Container-title:Metabolomics
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language:en
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Short-container-title:Metabolomics
Author:
Sander Katrin N., Kim Dong-Hyun, Ortori Catharine A., Warren Averil Y., Anyanwagu Uchenna C., Hay Daniel P., Broughton Pipkin Fiona, Khan Raheela N.ORCID, Barrett David A.
Abstract
Abstract
Introduction
Pre-eclampsia is a hypertensive gestational disorder that affects approximately 5% of all pregnancies.
Objectives
As the pathophysiological processes of pre-eclampsia are still uncertain, the present case–control study explored underlying metabolic processes characterising this disease.
Methods
Maternal peripheral plasma samples were collected from pre-eclamptic (n = 32) and healthy pregnant women (n = 35) in the third trimester. After extraction, high-resolution mass spectrometry-based untargeted metabolomics was used to profile polar and apolar metabolites and the resulting data were analysed via uni- and multivariate statistical approaches.
Results
The study demonstrated that the metabolome undergoes substantial changes in pre-eclamptic women. Amongst the most discriminative metabolites were hydroxyhexacosanoic acid, diacylglycerols, glycerophosphoinositols, nicotinamide adenine dinucleotide metabolites, bile acids and products of amino acid metabolism.
Conclusions
The putatively identified compounds provide sources for novel hypotheses to help understanding of the underlying biochemical pathology of pre-eclampsia.
Funder
British Heart Foundation
Publisher
Springer Science and Business Media LLC
Subject
Clinical Biochemistry,Biochemistry,Endocrinology, Diabetes and Metabolism
Reference39 articles.
1. Bahado-Singh, R. O., Akolekar, R., Mandal, R., Dong, E., Xia, J., Kruger, M., et al. (2012). Metabolomics and first-trimester prediction of early-onset preeclampsia. Journal of Maternal-Fetal and Neonatal Medicine, 25, 1840–1847. 2. Bahado-Singh, R. O., Akolekar, R., Mandal, R., Dong, E., Xia, J., Kruger, M., et al. (2013). First-trimester metabolomic detection of late-onset preeclampsia. American Journal of Obstetrics and Gynecology, 208, 58.e1–58.e7. 3. Chan, E. C. Y., Pasikanti, K. K., & Nicholson, J. K. (2011). Global urinary metabolic profiling procedures using gas chromatography-mass spectrometry. Nature Protocols, 6, 1483–1499. 4. De Oliveira, L., Câmara, N. O. S., Bonetti, T., LoTurco, E. G., Bertolla, R. P., Moron, A. F., et al. (2012). Lipid fingerprinting in women with early-onset preeclampsia: A first look. Clinical Biochemistry, 45, 852–855. 5. Dunn, W. B., Broadhurst, D. I., Atherton, H. J., Goodacre, R., & Griffin, J. L. (2011). Systems level studies of mammalian metabolomes: The roles of mass spectrometry and nuclear magnetic resonance spectroscopy. Chemical Society Reviews, 40, 387–426.
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