Oxidative status in Medicago truncatula Gaertn. non-embryogenic and embryogenic tissues with particular reference to somatic embryogenesis

Author:

Orłowska Anna,Kępczyńska Ewa

Abstract

Abstract Abiotic stress conditions (e.g., wounding, sterilization) are often together with plant growth regulators (e.g., 2,4-d), considered as one of the most important factors initiating plant somatic embryogenesis (SE). The first goal of this work was to answer whether leaf explants of the Medicago truncatula non-embryogenic (M9) line and its embryogenic variant (M9-10a) respond equally to mechanical and chemical stress by analyzing ROS (reactive oxygen species, e.g., O2•−, H2O2) localization, ROS scavenging enzyme activity and expression of genes encoding these enzymes. In explants of both lines, the stress response induced by wounding and chemical sterilization and the defense reaction during the 1st week of callus growth was similar. These defense mechanisms first involve an increase in SOD and CAT activity, later APX. 2,4-d, present at a low concentration (0.5 µM) during the induction phase (IP), is necessary for embryogenic callus formation and, consequently, for embryo development. This herbicide in higher concentrations causes an increase in O2•− accumulation and in antioxidant enzyme activity; however, it does not block the formation of callus and somatic embryos, though it disturbs these processes. Moreover, inhibition or blocking studied processes by DPI, an inhibitor of NADPH oxidase responsible for the production of O2•− and also lowering the expression of genes encoding the antioxidant enzymes leading to change in their activities, clearly indicate that a certain level of ROS is necessary to induce SE.

Publisher

Springer Science and Business Media LLC

Subject

Horticulture

Reference38 articles.

1. Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York

2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

3. Cross AR, Jones OT (1986) The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labeling of a component polypeptide of the oxidase. Biochem J 237(1):111–116

4. Elhiti M, Stasolla C (2015) ROS signalling in plant embryogenesis. In: Gupta K, Igamberdiev A (eds) Reactive oxygen and nitrogen species signaling and communication in plants. Signaling and communication in plants, vol 23. Springer, Cham

5. Fehér A (2015) Somatic embryogenesis—stress-induced remodeling of plant cell fate. Biochim Biophys Acta 1849(4):385–402

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3