Protonema induction, transient transformation, and protoplast regeneration in the peat moss Sphagnum papillosum

Abstract

AbstractSphagnum mosses are important carbon sequesters and emerging model organisms. However, induction and long-term cultivation of thalloid protonema in several species was not achievable so far. Here, we provide protocols for a set of new tools relevant for Sphagnum molecular biology: a new way for Sphagnum protoplast isolation and regeneration, and a first protocol for transient protoplast transformation. Together, these protocols will support the emerging Sphagnum research community in basic and applied science.