Affiliation:
1. The Laboratory of Experimental Cell Therapeutics, Geneva University Medical School CH-1211, Geneva 4, Switzerland
Abstract
In the present work combined glycan-, lectin-, and immunoblotting of isolated brain and kidney membranes shows that the α and β subunits of Na,K-ATPase are the most abundant glycoproteins. Further,Datura stramonium and Galanthus nivalis agglutinins recognize the Na,K-ATPase subunits in a mutually exclusive manner in membranes from human, rabbit and rat brain or human, rabbit, rat, pig and dog kidney indicating the presence of species-independent organ-typical glycoforms. The glycosylation status is not related to the ouabain-sensitivity. Taken together, the data reveals organ-specific glycoforms of Na,K-ATPase which might have roles for organ identification and recognition.
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
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