High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium

Author:

Tokashiki Michiyuki,Arai Takami

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Clinical Biochemistry,Biomedical Engineering,Bioengineering,Biotechnology

Reference8 articles.

1. Duff RG (1985) Microencapsulation technology: a novel method for monoclonal antibody production. Tends in Biotechnology 3: 167?171.

2. Himmelfarb P, Thayer PS and Martin HE (1969) Spin filter culture: The propagation of mammalian cells in suspension. Science 164: 555?557.

3. Knazek RA, Gullino PM, Kohler PO and Dedrick RL (1972) Cell culture on artificial capillaries. An approach to tissue growth in vitro. Science 178: 65?67.

4. Lydersen K, Pugh G, Paris S, Sharma P and Noll A (1985) Ceramic matrix for large scale animal cell culture. Bio/Technology 3: 63?67.

5. Murakami H, Shimomura T, Nakamura T, Ohashi H, Shinohara K and Omura H (1984) Development of a basal medium for serum-free cultivation of hybridoma cells in high density. J. Agric. Chem. Soc. Japan, 58: 575?583.

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