Generation of Multicellular Breast Cancer Tumor Spheroids: Comparison of Different Protocols

Author:

Froehlich Karolin,Haeger Jan-Dirk,Heger Julia,Pastuschek Jana,Photini Stella Mary,Yan Yan,Lupp Amelie,Pfarrer Christiane,Mrowka Ralf,Schleußner Ekkehard,Markert Udo R.,Schmidt André

Funder

Wilhelm Sander-Stiftung (DE)

Evangelisches Studienwerk Villigst (DE)

German Academic Exchange Service (DE)

Thüringer Ministerium für Bildung, Wissenschaft und Kultur (DE)

Publisher

Springer Science and Business Media LLC

Subject

Cancer Research,Oncology

Reference28 articles.

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2. Mehta G et al. Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy. J Control Release. 2012;164(2):192–204.

3. Nederman T et al. Demonstration of an extracellular matrix in multicellular tumor spheroids. Cancer Res. 1984;44(7):3090–7.

4. Rotin D, Robinson B, Tannock IF. Influence of hypoxia and an acidic environment on the metabolism and viability of cultured cells: potential implications for cell death in tumors. Cancer Res. 1986;46(6):2821–6.

5. Nagelkerke A et al. Generation of multicellular tumor spheroids of breast cancer cells: how to go three-dimensional. Anal Biochem. 2013;437(1):17–9.

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