Simultaneous demonstration of two antigens in ultrathin cryosections by a novel application of an immunogold staining method using primary antibodies from the same species

Author:

Bastholm L.,Nielsen M. H.,Larsson L. -I.

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Medicine,Anatomy,Cell Biology,Medical Laboratory Technology,Molecular Biology,Histology

Reference10 articles.

1. Bastholm L, Scopsi L, Nielsen MH (1986) Silver-enhanced immunogold staining of semithin and ultrathin cryosections. J Electron Microsc Tech 4:175?176

2. Bendayan M, Stephens H (1984) Double labelling cytochemistry applying the protein A gold technique. In: Polak JM, Varndell IM (eds) Immunolabelling for electron microscopy. Elsevier North-Holland, Amsterdam, pp 143?154

3. Geuze HJ, Slot JW, Scheffer RCT, van der Ley PA (1981) Use of colloidal gold particles in double-labelling immuno-electron microscopy of ultrathin frozen sections. J Cell Biol 89:653?665

4. Griffiths G, McDowell A, Back R, Dubochet J (1984) On the precipitation of cryosections for immunocytochemistry. J Ultrastruct Res 89:65?84

5. Slot JW, Geuze HJ (1984) Gold markers for single and double immunolabelling of ultrathin cryosections. In: Polak JM, Varndell IM (eds) Immunolabelling for electron microscopy. Elsevier North-Holland, Amsterdam, pp 129?142

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