Author:
Franken Sebastian,Bussmann Hendrik,Häberlein Hanns,Bonke Erik,End Caroline
Abstract
AbstractSingle-particle tracking (SPT) is a method of high-resolution microscopy to investigate the dynamics of single molecules inside cells or on the cell surface. Here we describe for the first time the applicability of the HiBiT Protein Tagging System combined with the HaloTag® self-labeling protein technology for monitoring the lateral diffusion of a pharmacological relevant G protein-coupled receptor (GPCR) by SPT.
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Biotechnology