Author:
Gottwald Eric,Grün Christoph,Liebsch Gregor
Abstract
AbstractOxygen is a crucial parameter for organotypic in vitro-cultures which are still performed under ambient atmosphere supplemented with 5 % CO2. This can lead to hyperoxia with its associated effects on signaling cascades and their effects on cellular behavior. Here, we describe a platform that enables real-time, label-free, optical oxygen (gradient) measurements in organotypic 3D-/organoid cultures thus allowing for physioxic culture and assay conditions in e. g. mito stress tests and substance testing.
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Biotechnology
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