Das FuN Screen-Prinzip zur experimentellen Analyse von Nanoporen in E. coli

Abstract

AbstractNanopores constitute an important class of biotechnologically relevant proteins. Unlike binders and enzymes, their experimental characterization is limited to high-resolution, yet low throughput biophysical methods. Addressing this technological gap, the functional nanopore (FuN) screen now provides a versatile assay to study and engineer nanopores in Escherichia coli combining quantitative resolution with the ease, scalability, and throughput of cellular assays.