Quantifizierung von Transkription und Translation von Riboschaltern-Reference-Cited by-同舟云学术

Quantifizierung von Transkription und Translation von Riboschaltern

Published:2024-05 Issue:3 Volume:30 Page:292-295
ISSN:0947-0867
Container-title:BIOspektrum
language:de
Short-container-title:Biospektrum

Author:

Martin Maria Wirtz,Wacker Anna,Schwalbe Harald

Abstract

AbstractRiboswitches change their secondary structure upon ligand binding, sending a signal that triggers transcriptional or translational gene regulation. Our dual-readout assay allows the simultaneous detection of ligand-dependent changes in transcription and translation. The cell-free assay utilizes the parallel detection of the fluorescent Mango (IV) aptamer downstream of the transcribed mRNA and GFP fluorescence to quantify transcription and translation products in the presence or absence of ligand.

Publisher

Springer Science and Business Media LLC

Link

https://link.springer.com/content/pdf/10.1007/s12268-024-2198-6.pdf

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