A cryptic variation in a member of the Ovate Family Proteins is underlying the melon fruit shape QTL fsqs8.1
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Published:2021-11-25
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ISSN:0040-5752
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Container-title:Theoretical and Applied Genetics
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language:en
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Short-container-title:Theor Appl Genet
Author:
Martínez-Martínez Cecilia, Gonzalo Maria José, Sipowicz Pablo, Campos Manuel, Martínez-Fernández Irene, Leida Carmen, Zouine Mohammed, Alexiou Konstantinos G., Garcia-Mas Jordi, Gómez María Dolores, Tornero Pablo, Pérez-Amador Miguel Ángel, Esteras Cristina, Picó Belén, Romero Carlos, Monforte Antonio J.ORCID
Abstract
Abstract
Key message
The gene underlying the melon fruit shape QTL fsqs8.1 is a member of the Ovate Family Proteins. Variation in fruit morphology is caused by changes in gene expression likely due to a cryptic structural variation in this locus.
Abstract
Melon cultivars have a wide range of fruit morphologies. Quantitative trait loci (QTL) have been identified underlying such diversity. This research focuses on the fruit shape QTL fsqs8.1, previously detected in a cross between the accession PI 124112 (CALC, producing elongated fruit) and the cultivar ‘Piel de Sapo’ (PS, producing oval fruit). The CALC fsqs8.1 allele induced round fruit shape, being responsible for the transgressive segregation for this trait observed in that population. In fact, the introgression line CALC8-1, carrying the fsqs8.1 locus from CALC into the PS genetic background, produced perfect round fruit. Following a map-based cloning approach, we found that the gene underlying fsqs8.1 is a member of the Ovate Family Proteins (OFP), CmOFP13, likely a homologue of AtOFP1 and SlOFP20 from Arabidopsis thaliana and tomato, respectively. The induction of the round shape was due to the higher expression of the CALC allele at the early ovary development stage. The fsqs8.1 locus showed an important structural variation, being CmOFP13 surrounded by two deletions in the CALC genome. The deletions are present at very low frequency in melon germplasm. Deletions and single nucleotide polymorphisms in the fsqs8.1 locus could not be not associated with variation in fruit shape among different melon accessions, what indicates that other genetic factors should be involved to induce the CALC fsqs8.1 allele effects. Therefore, fsqs8.1 is an example of a cryptic variation that alters gene expression, likely due to structural variation, resulting in phenotypic changes in melon fruit morphology.
Funder
Ministerio de Economía y Competitividad Ministerio de Ciencia e Innovación Conselleria d’Educació, Investigació, Cultura i Esports Ministerio de Educación Consejo Superior de Investigaciones Cientificas
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Agronomy and Crop Science,General Medicine,Biotechnology
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