Abstract
AbstractThe effect of chloramphenicol, an often used protein synthesis inhibitor, in photosynthetic systems was studied on the rate of Photosystem II (PSII) photodamage in the cyanobacterium Synechocystis PCC 6803. Light-induced loss of PSII activity was compared in the presence of chloramphenicol and another protein synthesis inhibitor, lincomycin, by measuring the rate of oxygen evolution in Synechocystis 6803 cells. Our data show that the rate of PSII photodamage was significantly enhanced by chloramphenicol, at the usually applied 200 μg mL−1 concentration, relative to that obtained in the presence of lincomycin. Chloramphenicol-induced enhancement of photodamage has been observed earlier in isolated PSII membrane particles, and has been assigned to the damaging effect of chloramphenicol-mediated superoxide production (Rehman et al. 2016, Front Plant Sci 7:479). This effect points to the involvement of superoxide as damaging agent in the presence of chloramphenicol also in Synechocystis cells. The chloramphenicol-induced enhancement of photodamage was observed not only in wild-type Synechocystis 6803, which contains both Photosystem I (PSI) and PSII, but also in a PSI-less mutant which contains only PSII. Importantly, the rate of PSII photodamage was also enhanced by the absence of PSI when compared to that in the wild-type strain under all conditions studied here, i.e., without addition and in the presence of protein synthesis inhibitors. We conclude that chloramphenicol enhances photodamage mostly by its interaction with PSII, leading probably to superoxide production. The presence of PSI is also an important regulatory factor of PSII photodamage most likely via decreasing excitation pressure on PSII.
Funder
Hungarian Ministry for National Economy
Hungarian Ministry for National Economy orschung und Wirtschaft
ELKH Biological Research Center
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Plant Science,Biochemistry,General Medicine
Reference54 articles.
1. Aikens J, Dix TA (1991) Perhydroxyl radical (HOO.) initiated lipid-peroxidation—the role of fatty-acid hydroperoxides. J Biol Chem 266(23):15091–15098
2. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
3. Arntzen CJ, Kyle DJ, Wettern M, Ohad I (1984) Photoinhibition: a consequence of the accelerated breakdown of the apoprotein of the secondary electron acceptor of Photosystem II. In: Hallick R, Staehelin LA, Thornber JP (eds) Biosynthesis of the photosynthetic apparatus: molecular biology development and regulation. UCLA Symposium Series, New York, pp 313–324
4. Aro E-M, Virgin I, Andersson B (1993) Photoinhibition of Photosystem II. Inactivation, protein damage and turnover. Biochim Biophys Acta 1143:113–134
5. Baena-Gonzalez E, Aro E-M (2002) Biogenesis, assembly and turnover of Photosystem II units. Phyl Trans R Soc Lond B 357:1451–1460
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