Author:
Kim Jong-Heun,Baek Jeong-In,Lee In-Kyu,Kim Un-Kyung,Kim Ye-Ri,Lee Kyu-Yup
Abstract
Abstract
Background
Cisplatin (CP) is an effective anticancer drug broadly used for various types of cancers, but it has shown ototoxicity that results from oxidative stress. Berberine has been reported for its anti-oxidative stress suggesting its therapeutic potential for many diseases such as colitis, diabetes, and vascular dementia.
Objective
Organ of Corti of postnatal day 3 mouse cochlear explants were used to compare hair cells after the treatment with cisplatin alone or with berberine chloride (BC) followed by CP.
Methods
We investigated the potential of the anti-oxidative effect of BC against the cisplatin-induced ototoxicity. We observed a reduced aberrant bundle of stereocilia in hair cells in CP with BC pre-treated group. Caspase-3 immunofluorescence and TUNEL assay supported the hypothesis that BC attenuates the apoptotic signals induced by CP. Reactive oxygen species level in the mitochondria were investigated by MitoSOX Red staining and the mitochondrial membrane potentials were compared by JC-1 assay.
Results
BC decreased ROS generation with preserved mitochondrial membrane potentials in mitochondria as well as reduced DNA fragmentation in hair cells. In summary, our data indicate that BC might act as antioxidant against CP by reducing the stress in mitochondria resulting in cell survival.
Conclusion
Our result suggests the therapeutic potential of BC for prevention of the detrimental effect of CP-induced ototoxicity.
Funder
national research foundation of korea
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,Biochemistry
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