Development of markers using microsatellite loci of two rove beetle species, Paederus fuscipes Curtis and Aleochara (Aleochara) curtula Goeze (Coleoptera: Staphylinidae), followed by analyses of genetic diversity and population structure

Author:

Choi Yeon‐Jae,Yi Jeesoo,Lee Chan-Jun,Kim Ji-Wook,Jeon Mi-Jeong,Park Jong-Seok,Cho Sung-JinORCID

Abstract

Abstract Background The family Staphylinidae is the most speciose beetle group in the world. The outbreaks of two staphylinid species, Paederus fuscipes and Aleochara (Aleochara) curtula, were recently reported in South Korea. None of research about molecular markers and genetic diversity have been conducted in these two species. Objective To develop microsatellite markers and analyze the genetic diversity and population structures of two rove beetle species. Methods NGS was used to sequence whole genomes of two species, Paederus fuscipes and Aleochara (Aleochara) curtula. Microsatellite loci were selected with flanking primer sequences. Specimens of P. fuscipes and A. curtula were collected from three localities, respectively. Genetic diversity and population structure were analyzed using the newly developed microsatellite markers. Results The number of alleles ranged 5.727–6.636 (average 6.242) and 2.182–5.364 (average 4.091), expected heterozygosity ranged 0.560–0.582 (average 0.570) and 0.368–0.564 (average 0.498), observed heterozygosity ranged 0.458–0.497 (average 0.472) and 0.418–0.644 (average 0.537) in P. fuscipes and A. curtula, respectively. Population structure indicates that individuals of A. curtula are clustered to groups where they were collected, but those of P. fuscipes are not. Conclusion Population structures of P. fuscipes were shallow. In A. curtula, however, it was apparent that the genetic compositions of the populations are different significantly depending on collection localities.

Funder

National Research Foundation of Korea

National Institute of Biological Resources

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Molecular Biology,Biochemistry

Reference28 articles.

1. Anlaş S, Çevik IE (2008) Faunistic studies on Paederinae (Coleoptera: Staphylinidae) in Manisa province, Turkey. Mun Ent Zool 3:665–674

2. Assing V (2020) On the taxonomy and zoogeography of Paederus V. Two new species from Laos and China, a new synonymy, new subgeneric assignments, and new records from the Palaearctic region (Coleoptera: Staphylinidae: Paederinae). Acta Mus Morav Sci Biol 105:91–102

3. Biswas MK, Bagchi M, Biswas D, Harikrishna JA, Liu Y, Li C, Sheng O, Mayer C, Yi G, Deng G (2020) Genome-wide novel genic microsatellite marker resource development and validation for genetic diversity and population structure analysis of banana. Genes 11:1479

4. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

5. Earl DA, vonHoldt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361

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