Abstract
AbstractThe parasitic guava root-knot nematode, Meloidogyne enterolobii Yang and Eisenback (syn. M. mayaguensis), has a wide distribution, host range and is established majorly in tropical-subtropical climates. A routine diagnostic analysis of symptomatic plant roots confirmed the first detection of M. enterolobii in Australia, near Darwin, Northern Territory. The morphology of female perineal patterns, second stage juveniles (J2s) and males were consistent with other reports of this species. The presence of M. enterolobii was first evaluated with a species-specific sequence-characterized amplified region (SCAR) and the intergenic spacer region (IGS2), followed by sequencing of the mitochondrial cytochrome c oxidase I and II (COI & COII) and large subunit ribosomal RNA (28S rRNA) gene regions. The nematode was detected on several important crop-host plants in Australia’s Northern Territory including chilli, tomato, capsicum, cucumber, pumpkin, eggplant, snake bean and sweet potato. The ability of M. enterolobii to colonise a variety of weeds and native Australian plants makes management challenging. The route of introduction of M. enterolobii is presently unknown but is under investigation.
Funder
Northern Territory Government
Commonwealth Scientific and Industrial Research Organisation
Publisher
Springer Science and Business Media LLC
Reference9 articles.
1. Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 54:165–173. https://doi.org/10.1016/0166-6851(92)90109-W
2. Hartman KM, Sasser JN (1985) Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. An Advanced Treatise on Meloidogyne. Volume II. Methodology. pp 69–77
3. edition;DJ Hooper,1986
4. Long H, Liu H, Xu JH (2006) Development of a PCR diagnostic for the root-knot nematode Meloidogyne enterolobii. Acta Phyto Sin 36:109–115
5. Nunn GB (1992) Nematode molecular evolution: an investigation of evolutionary patterns among nematodes based upon DNA sequences. Ph.D. Thesis. University of Nottingham, Nottingham, UK