High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris

Author:

Cai Huawei,Chen Lihong,Wan Lin,Zeng Lingyu,Yang Hao,Li Shengfu,Li Youping,Cheng Jingqiu,Lu Xiaofeng

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Reference28 articles.

1. Alegre ML, Fallarino F (2006) Mechanism of CTLA4-Ig in tolerance induction. Curr Pharm Des 12:149–160

2. Amoura Z, Piette JC (2006) New therapies for autoimmune diseases. Rev Neurol (Paris) 162(1):3S7–3S11

3. Chen LH, Huang Q, Wan L, Zeng LY, Li SF, Li YP, Lu XF, Cheng JQ (2006) Expression, purification, and in vitro refolding of a humanized single-chain Fv antibody against human CTLA4 (CD152). Protein Expr Purif 46:495–502

4. Clare JJ, Rayment FB, Ballantyne SP, Sreerkrishna K, Romanos MA (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Bio/Technology 9:455–460

5. Clarkson MR, Sayegh MH (2005) T-cell costimulatory pathways in allograft rejection and tolerance. Transplantation 80(5):555–63

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