Co-amplification of EBNA-1 and PyLT through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in CHO cells-Reference-Cited by-同舟云学术

Co-amplification of EBNA-1 and PyLT through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in CHO cells

Published:2018-04-13 Issue:11 Volume:102 Page:4729-4739
ISSN:0175-7598
Container-title:Applied Microbiology and Biotechnology
language:en
Short-container-title:Appl Microbiol Biotechnol

Author:

Lee Joo-Hyoung,Park Jong-Ho,Park Sun-Hye,Kim Sun-Hong,Kim Jee Yon,Min Jeong-Ki,Lee Gyun Min,Kim Yeon-Gu

Funder

A grant from the National Research Foundation of Korea (NRF) funded by the Korea government

A grant from KRIBB Initiative Program

Publisher

Springer Science and Business Media LLC

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

http://link.springer.com/article/10.1007/s00253-018-8977-6/fulltext.html

Reference34 articles.

1. Backliwal G, Hildinger M, Chenuet S, Wulhfard S, Jesus MD, Wurm FM (2008) Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic Acids Res 36(15):e96

2. Brummelkamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296(5567):550–553

3. Codamo J, Munro TP, Hughes BS, Song M, Gray PP (2011) Enhanced CHO cell-based transient gene expression with the epi-CHO expression system. Mol Biotechnol 48(2):109–115

4. Daramola O, Stevenson J, Dean G, Hatton D, Pettman G, Holmes W, Field R (2014) A high-yielding CHO transient system: coexpression of genes encoding EBNA-1 and GS enhances transient protein expression. Biotechnol Prog 30(1):132–141

5. Dumont J, Euwart D, Mei B, Estes S, Kshirsagar R (2016) Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives. Crit Rev Biotechnol 36(6):1110–1122

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2. Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system;Applied Microbiology and Biotechnology;2020-11-16

3. Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins;Journal of Bioscience and Bioengineering;2019-10

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